【Abstract】 Objective To understand the pattern of epidemic areas of hemorrhagic fever with renal syndrome (HFRS) in Fujian and to provide scientific basis for its prevention and control.  Methods Rats were captured in springs and autumns by live snap method, and its density and specie composition were investigated. The virus antigen from rodent lungs was isolated and identified. Results The dominant species in residential areas was Rattus norvegicus in Rattus type areas and the mixed?type area, which density were 4.91% and 5.73% at home respectively. While in the field, it was Apodemus agrarius with the average density of 12.95%, which was obviously higher in autumn and winter than that in spring. R.norvegicus mainly carried with Type Ⅱ virus, and Ap.agrarius with Type Ⅰvirus. A44 and R50 virus strain were isolated from them, respectively. Conclusion R.norvegicus was still the main host of HFRS in Fujian. Ap.agrarius was the main host in the field in the diversity epidemic area. It should strengthen the surveillance and the control of HFRS in main epidemic areas.

Objective To establish a sensitive and specific method for detecting total antibodies in sera of hemorrhagic fever with renal syndrome(HFRS) patients and rodents by the double antigens sandwich ELISA. Methods The double antigens sandwich ELISA was established with coating antigen e1.3S from Hantavirus (HV), combining with recombinant antigen e6-119 labeled with HRP. The method was used to detect total antibodies in sera of HFRS patients and rodents, and the results were compared to that of indirect fluorescent assay(IFA). Results Five hundred and sixty-six samples (188 human sera and 378 rodents sera) were detected by using the double antigens sandwich ELISA and IFA. The total coincidence rate of the results by the two methods was 97.70%. Compared with IFA, the ELISA for the detection of total antibodies showed 97.54% in sensitivity and 97.87% in specificity. Conclusion The double antigens sandwich ELISA showed higher sensitivity and specificity for the detection of total antibodies, which can be applied in the large-scale epidemiologic survey.